OYEKALE Solomon

Pan African University Institute of Life and Earth Sciences(PhD)

Research Topic

Genetic analyses of Striga resistance and tolerance to low soil nitrogen in crosses of extra-early provitamin A quality protein maize (Zea mays L.) inbred lines in Nigeria

Research Description

Striga hermonthica parasitism and Low-N significantly constrain maize production and productivity in sub-Saharan Africa (SSA). The normal maize, use as staple food in SSA, is deficient in provitamin-A, tryptophan and lysine, predisposing millions of people to vitamin A deficiency (VAD) and protein malnutrition. The main objective of this study was to develop provitamin-A quality protein maize hybrids that would be high-yielding and stable in performance across Striga and Low-N environments. It is hoped that the hybrids will be released, after further testing in on-farm trials, to combat the problems of Striga, Low-N, VAD and protein malnutrition in SSA.


Beuken Van-den-Rob

Wageningen University & Research (M.Sc.)

Research Topic

Water availability and potassium uptake of cassava

Research Description

This research is focussed on the role of potassium within in the cassava crop. Are cassava plants which are extracting a lot of potassium from the soil also able to be more drought resistance? This is tested by looking into the hydrological aspects of the field and factors influencing water use efficiency of the crop like rooting patterns and stomatal regulation.


Eleta Valentine

Pan African University, Institute of Life & Earth Sciences (PhD)

Research Topic

Fertigation and Water Management in Cassava Farming Systems

Research Description

The Research is geared towards assessing the growth and root yield responses of some cassava varieties to different irrigation and fertiliser application regimes.


Lawal Oluwafemi

University of Ilorin, Ilorin (PhD)

Research Topic

Genetic analysis of maize inbred lines under low N environments in Nigeria

Research Description

The research aims to breed low N tolerant maize intended to address perennial problem of low soil N in Nigeria in addition to high cost of N fertilizer usually outside the reach of farmers and environmental issues associated with N use and abuse.


SULLEY MARCO

PAN AFRICAN UNIVERSITY, UNIVERSITY OF IBADAN (M.Sc.)

Research Topic

Development of storage roots in cassava (Manihot esculenta): involved gene expression validation.

Research Description

"Cassava has one of the highest rates of CO2 fixation and sucrose synthesis for any C3 plant, but rarely reaches its yield potentials in the field. It was the hypothesis that starch production in cassava storage roots can be increased substantially by increasing the sink strength for carbohydrate.Previous studies have been done to investigate the gene potential for storage root bulking in cassava but the time point where these gene are expressed are not clearly known to improve the cassava variety selection for high yielding. Due to revolution in molecular biology techniques reverse transcription quantitative PCR (RT-qPCR) is considered today as the gold standard for accurate, sensitive and fast measurement of gene expression. This study aims to unlock the genetic potential for starch mobility in cassava at specific time."


Ofoyeju Bertha

Pan African University(M.Sc.)

Research Topic

Evaluation of sets of recombinant inbred lines of cowpea under drought tolerance

Research Description

"A sets of recombinant inbred lines of cowpea from University of California, Riverside will be evaluated under drought and irrigated conditions and the traits to consider are days to flowering, leaf color using SPAD meter, virus scoredays to 50 days maturity, pod number perplants, pod weight per plant, pod stand per plot at harvest, 100 seed weight per plant and seed thickness. In conclusion all the traits taken will be evaluated using ANOVA and SAS version 12... Recommendations will be made based on the results of the analysis "


Shirima Rudolph

University of Dar es Salaam (PhD )

Research Topic

Cassava Brown Streak ipomoviruses (CBSIs) interactions

Research Description

CBSIs cause cassava brown streak disease (CBSD) which makes the roots inedible and unmarketable by causing dry necrotic rots. CBSD causes major economic losses to farmers in East and Central Africa. Lack of resistant varieties makes it necessary to increase search for resistance and to strengthen efforts to mitigate further spread. Performance of different cassava varieties under diverse agro-ecologies and virus inoculum pressure will be investigated. The effects of CBSD on cassava degeneration will be evaluated. A diagnostic tool for measuring virus titre has been developed to enable researcher to compare the level of virus resistance depicted by different varieties


Adediji Adedapo

University of Ibadan (PhD )

Research Topic

DIVERSITY AND IMPROVED DIAGNOSIS OF Banana bunchy top virus (Nanoviridae: Babuvirus) ON Musa SPECIES IN SOUTHWESTERN NIGERIA

Research Description

Banana (Musa spp.) is an important source of food but its production is limited by Banana bunchy top virus (BBTV), vectored by banana aphid, Pentalonia nigronervosa (PN). Information on characteristics and detection of BBTV in southwestern Nigeria is limited. Therefore, the genetic diversity and improved methods of BBTV diagnosis were investigated. Banana leaf samples were obtained from four states: Lagos, Ogun, Oyo and Kwara. Disease incidence and severity were determined. Full length of each BCs (DNA-R, DNA-U3, DNA-S, DNA-M, DNA-C and DNA-N) were sequenced. Primers were designed for loop-mediated isothermal amplification (LAMP) and PCR to target DNA-N and compared with DNA-R primers. Direct-sap PCR [DS-PCR], direct-sap LAMP [DS-LAMP], direct-binding PCR [DB-PCR] and direct-binding LAMP [DB-LAMP] were assessed for sensitive detection of BCs. TaqMan® qPCR was used to quantify BCs by Standard Curves (SC) and linear correlation. CT of BCs were obtained from banana leaves and PN. 15.3% of leaf samples were positive for BBTV from Ogun State. Virus incidence and severity ranged from 5.6% to 78.6% and 1.2 to 2.1, respectively. Isolates had ≥99.5% nucleic acid and ≥97.6% amino acid similarities. The sensitivities of DNA-N primers were 1,000.0% higher than DNA-R-based primers. Detection limit was 1.0ng/µL for DB-PCR and DS-PCR, while DB-LAMP, DS-LAMP and PCR were sensitive at 100.0pg/µL. LAMP assay for DNA-N was the most sensitive at 100.0fg/µL. The qPCR assays detected BBTV from 0.7 DNA-N to 3.1 DNA-S copies. Across the BCs, SC correlations were positive, from 0.99 to 1.00. Genetic diversity of BBTV in southwestern Nigeria was low. The use of direct-sap loop-mediated isothermal amplification was the best method for direct virus identification in bananas.


SHONDE Temitope

Ladoke Akintola University of Technology(M.Sc.)

Research Topic

DIVERSITY ASSESSMENT OF WINGED BEAN (Psophocarpus tetragonolobus (L.) DC.) ACCESSIONS USING MORPHOLOGICAL TRAITS AND MICROSATELLITES.

Research Description

Generating suitable parental lines would help to accelerate winged bean improvement through selection and hybridization programs. It is necessary to understand the diversity existing among available accessions. Aim of this study, are to assess (i) the morphological variation within 15 winged bean accessions, and (ii) the genetic diversity within the accessions using microsatellite primers.


Hanmakyugh Teryima Teddy

University of Ibadan(M.Sc.)

Research Topic

Qualitative and Quantitative study of inbred genetic stocks of Cassava (Manihot esculenta Crantz)

Research Description

"Cassava (Manihot esculenta Crantz) is among the most important sources of energy in the diet of millions, particularly in Sub-Saharan Africa. Recently its importance and demand have evolved to become a cash and industrial crop having unique traits and characteristics such as starch, high protein, dry matter content and high carotene content. To address these demands, breeders seek to surmount the constraints associated with its breeding scheme which includes the length of the breeding cycle and its heterozygous nature. Cassava genetic stocks represent a unique and expanding class of extremely valuable germplasm extensively used in the improvement of cassava for maximizing gains for key traits. In this study, twenty three cassava genotypes and one castor bean plant of the genetic stocks polyploid population were screened by isolating DNA nuclei, to investigate ploidy levels with flow cytometry. Two hundred and twenty three inbred families representing different levels of inbreeding were also evaluated and characterized in a seedling nursery. Comparison of relative nuclear DNA content with flow cytometry revealed group cassava into different ploidy levels. In the seedling nursery trial variation was observed in diseases, root number and root weight among families. "

Contact Us

  • Address: IITA Headquarters, PMB 5320, Oyo Road, Ibadan 200001, Oyo State, Nigeria

  • Phone: (+234)806-435-3738

  • Email: iita-iarsaf@cgiar.org